타이타늄 표면에 코팅된 epigallocatechin gallate의 사람치수세포 분화 촉진
Epigallocatechin gallate coated on Ti surface stimulates differentiation of human dental pulp cells
김영석, 박희철, 이동희, 양형철,
소속 상세정보
김영석 ( Kim Young-Seok ) - 서울대학교 치의학대학원 치과생체재료과학교실
박희철 ( Park Hee-Chul ) - 서울대학교 치의학대학원 치과생체재료과학교실
이동희 ( Lee Dong-Hee ) - 서울대학교 치의학대학원 치과생체재료과학교실
양형철 ( Yang Hyeong-Cheol ) - 서울대학교 치의학대학원 치과생체재료과학교실
KMID : 1035320150420030197
Abstract
Epigallocatechin gallate (EGCG), a flavonoid which is found in green tea and red wine, is known to have an osteogenic activity. In this study, the effect of EGCG on the differentiation of human dental pulp cells (HDPCs) was investigated. The growth of HDPCs was suppressed when the cells were exposed to 60 μM EGCG during 5 days. EGCG enhanced ALP activity of HDPCs at concentrations ranging from 1 to 30 μM. Furthermore, the mRNA expression of osteocalcin (OCN) and dentin sialophosphoprotein (DSPP) in HDPCs was enhanced by exposure to EGCG. Extracellular matrix mineralization was also promoted by EGCG even in the absence of other odontogenic factors such as ascorbic acid and dexamethasone. Therefore, it is certain that EGCG is capable of inducing odontogenicity of dental pulp cells. In the experiment of biomimetic apatite coating of titanium discs, EGCG was successfully incorporated into apatite coats and released from the surfaces into culture media.
키워드
Epigallocatechin gallate; Human dental pulp cell; Differentiation; Dentin; Pulp
원문 및 링크아웃 정보
등재저널 정보